37 research outputs found
Recommended from our members
Regulatory cocktail for dopaminergic neurons in a protovertebrate identified by whole-embryo single-cell transcriptomics
The CNS of the protovertebrate Ciona intestinalis contains a single cluster of dopaminergic (DA) neurons, the coronet cells, which have been likened to the hypothalamus of vertebrates. Whole-embryo single-cell RNA sequencing (RNA-seq) assays identified Ptf1a as the most strongly expressed cell-specific transcription factor (TF) in DA/coronet cells. Knockdown of Ptf1a activity results in their loss, while misexpression results in the appearance of supernumerary DA/coronet cells. Photoreceptor cells and ependymal cells are the most susceptible to transformation, and both cell types express high levels of Meis Coexpression of both Ptf1a and Meis caused the wholesale transformation of the entire CNS into DA/coronet cells. We therefore suggest that the reiterative use of functional manipulations and single-cell RNA-seq assays is an effective means for the identification of regulatory cocktails underlying the specification of specific cell identities
カンキョウ ハイリョ ショウヒン ノ フキュウ タイヨウ ト モーダル ミックス
環境配慮商品といえども、環境配慮の側面だけではその普及の過程は平坦とはいえない。環境負荷の大きい自動車を基軸として、環境配慮商品としての普及に向けた取り組みを整理しながら、モーダルミックスの示唆する環境配慮社会の構築の枠組みを探っていく。The diffusion of products may not be just a matter of dealing with environmentally conscious products. A review of the diffusion process, especially concerning cars, whose environmental burden can be considerable, would be appropriate in helping to find framework suggested by modal mix, leading to an environmentally conscious society